Abstract
The occurrence of 2009 H1N1 influenza pandemic spread rapidly worldwide and caused more than 18,000 mortalities in 4 months. Recent WHO surveillance data have shown that H3N2 virus is the predominant circulating seasonal flu virus in many countries. Earlier research suggested that the reassortment of influenza ribonucleoprotein (RNP) complex genes (PB2, PB1, PA and NP) has a tremendous impact on polymerase activity and virus replication. This study evaluated the effects of the reassortment between the H1N1 PA gene and the local H3N2 strain (A/TW3446/02; 3446) on RNP activity, growth kinetics and virulence in mice. Compared with the wild-type 3446, the reassortants of PR8 PA/3446 and 2009 pH1N1 PA/3446 grew more efficiently in human A549 cells but retained the same virulence and pathological change as 3446 in mice inoculated with high viral load.
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