GZFLW Induces Apoptosis of Ectopic Endometrial Stromal Cells via Promoting VPS53 Protein Stability.

Ziyu Zhang,Yang Zou,Quan Zhang,Faying Liu,Ouping Huang,Huang Huang,Anwen Xiong,Yunyun Xu,Liqun Wang,Yong Luo,Bicheng Yang

doi:10.1155/2018/1293630

Abstract

Endometriosis is still a major problem in obstetrics and gynecology. While GZFLW (Gui Zhi Fu Ling Wan) has been originally used for treating gynecological diseases, however, the molecular mechanism that GZFLW acts on endometriosis is not clear. To investigate the molecular mechanism that GZFLW plays role on endometriosis, iTRAQ (isobaric tags for relative and absolute quantification) proteomics and human endometrial stromal cells (Y14) obtained from a patient with endometriosis were used in in vitro study. Our results demonstrated that GZFLW decreased Y14 cells proliferation while increased cells apoptosis. The differential expression protein VPS53 (Vacuolar protein sorting 53 homolog) was predicted by iTRAQ coupled LC-MS/MS and further identified by western blot. Besides, GZFLW induced VPS53 protein level by promoting its stabilization. Our findings highlight a novel role for VPS53 in gynecology and provide a potent therapeutic strategy against endometriosis.

Highlights

Endometriosis is the presence of endometrial cells in other parts outside the uterine cavity, such as ovaries, fallopian tubes, bladder, and myometrium [1]
While Gui Zhi Fu Ling Wan (GZFLW) has been originally used for the treatment of gynecological diseases [7], the molecular mechanism that GZFLW acts on endometriosis is not clear
Our results demonstrate that GZFLW can increase the expression of VPS53 through promoting its stability and induce the apoptosis of endometriosis stromal cells via mitochondria-induced apoptotic pathway

Summary

Introduction

Endometriosis is the presence of endometrial cells in other parts outside the uterine cavity, such as ovaries, fallopian tubes, bladder, and myometrium [1]. The main pathological changes are ectopic endometrial bleeding and the surrounding tissue fibrosis. The main symptoms of endometriosis are the formation of ectopic nodules, dysmenorrhea, chronic pelvic pain, abnormal menstruation, and infertility, which are seriously affecting the health and quality of life of young women [2,3,4,5,6]. There is a long history of traditional Chinese medicine, which has contributed to the prevention and treatment of various diseases. Gui Zhi Fu Ling Wan (GZFLW) is one of the formulations in the ancient Chinese medicine and is composed of five crude drugs, including Cinnamomi Cortex, Poria cocos, Moutan Cortex, Persicae Semen, and Paeoniae Radix. While GZFLW has been originally used for the treatment of gynecological diseases [7], the molecular mechanism that GZFLW acts on endometriosis is not clear

Methods

Results

Conclusion