Abstract
The guava tree (psidium guajava linn.) is commonly used not only as food but also as folk medicine. In our previous studies, we showed that oral administration of guava leaf extracts (GLE) had beneficial anti-obesity effects using metabolic syndrome model rats. However, we did not clarify molecular mechanism by which GLE administration leads to anti-obesity effect. This study was designed to evaluate the mechanism of anti-obesity by GLE using 3T3-L1 pre-adipocyte cell lines. We found that GLE significantly inhibited 3T3-L1 differentiation via down-regulation of adipogenic transcription factors and markers. Mitotic clonal expansion, which is essential for adipose differentiation, was also depressed in the early phase. Interestingly, GLE increased the phosphorylation of AMPK on 3T3-L1 cells and, by pretreatment with AMPK siRNA, the GLE treatment group showed restored adipocyte differentiation. In conclusion, these results showed that GLE is capable of inhibiting adipocyte differentiation via AMPK activation and therefore it may prevent obesity in vivo.
Highlights
The guava tree is a member of the Myrtaceae family, which ranges over tropical and subtropical countries
We showed that oral administration of guava leaf extract (GLE) had beneficial anti-obesity effects, preventing body weight gain, reducing adipose tissue weight and fatty liver, and improving insulin resistance using SHRSP.Z-Leprfa/IzmDmcr rats, which were established as a new model of metabolic syndrome [7]
The results showed that GLE inhibited the accumulation of lipid droplets in Confluent cells were exposed to a differentiation cocktail and GLE at the indicated concentrations (A) or cells were exposed to GLE (100 ug/mL) for the indicated time period (B)
Summary
The guava tree (psidium guajava linn.) is a member of the Myrtaceae family, which ranges over tropical and subtropical countries It is commonly used as food and as folk medicine with antioxidant, antispasmodic, anti-allergy, anti-inflammatory and antidiabetic properties using extracts from the fruit, leaf, bark or roots [1, 2]. It was revealed that PPAR is a transcription factor that is indispensable for the differentiation of fat cells, and it is considered that C/EBP is a transcription factor with an essential role in the development of insulin sensitivity Of these factors, adipocyte specific fatty acid binding protein (aP2) is a fatty acid binding protein that functions in fatty acid import, storage and export [8, 9]. These are used as adipogenic markers to detect mature adipocytes
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
