Growth-Phase-Dependent Pea Lectin Receptors on Rhizobium leguminosarum as determined by an Enzyme-Linked Lectin Binding Assay (ELBA)

Ineke A. M. van der Schaal,Trudy J. J. Logman,Clara L. Díaz,Jan W. Kijne

doi:10.1007/978-94-009-6923-0_221

Growth-Phase-Dependent Pea Lectin Receptors on Rhizobium leguminosarum as determined by an Enzyme-Linked Lectin Binding Assay (ELBA)

Ineke A. M. van der Schaal, Trudy J. J. Logman + Show 2 more

https://doi.org/10.1007/978-94-009-6923-0_221

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Publication Date: Jan 1, 1984

#Enzyme-Linked Lectin Binding Assay #Pea Lectin + Show 8 more

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Abstract

Agglutination of Rhizobium with pea lectin (PL) was restricted to pea nodulating strains and only occurred during a short period after exponential growth had stopped. Binding of PL to Rhizobium was observed with several species and was not restricted to a particular growth phase. Hot saline extraction of the cells removed their light microscope visible capsule and their PL binding capacities (Van der Schaal et al. 1983). We have developed an enzyme-linked lectin binding assay (ELBA) to be able to quantitate PL receptors in these extracts and in extracellular polysaccharides (EPS) of Rhizobium.

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