Growth responses and rubisco activity influenced by antibiotics and organic amendments used for stress alleviation in Lactuca sativa

Abstract

The global increase in the consumption of antibiotics has resulted in contamination of different ecosystems with severe implications on crop productivity. This study investigated the effects of ampicillin and ofloxacin on Lactuca sativa germination upon solution exposure and growth when cultivated in soils treated with three organic amendments (compost, rice husk and vermicompost). Two levels of both antibiotics 5 and 10 mg L−1 (for solution) or mg kg−1 (for soil) were tested in addition to the control. Results indicated that addition of compost significantly (p < 0.05) increased (50%) the root lengths of plant exposed to ampicillin (5 mg L−1). Similarly, vermicompost-amended treatments displayed a 64% increase (p < 0.05) in the shoot length of seedlings under the effect of 5 mg L−1 ofloxacin, depicting a positive synergistic effect between the antibiotics and amendments in the germination test. Nevertheless, the germination percentage remained unaffected in all the treatments. In greenhouse experiment, enhanced plant biomass was observed with the use of rice husk across all the treatment groups. Comparable to the germination test, plants treated with rice husk and compost signaled a higher content of rubisco large subunit (157% and 85%, respectively) and soluble protein (248% and 108%, respectively) post antibiotics application. On the contrary, an antagonistic effect of the rice husk and ofloxacin 5 mg kg−1 was observed on the chlorophyll content, evident by a 37% decrease. Overall, it was observed that the effect of antibiotics on different plant traits vary depending on the antibiotic concentration as well as type of amendment used.