Growth of the chicken embryo: Implications of egg size

Abstract

Among avian species with large differences in egg size, changes in eggshell conductance and incubation time permit the water loss necessary for embryonic development. To what extent this happens for different-size eggs within a species is much less known. Chicken eggs with fresh egg weight (Wegg) either large (L,∼66g) or small (S,∼51g) were incubated at 38°C and 60% humidity; their yolk and albumen scaled almost in proportion to Wegg. Eggshell gas conductance scaled to 0.77 of Wegg, as it occurs inter-specifically, while external pipping and hatching occurred at similar times in S and L. Hence, L lost less water during incubation than S, and embryos of L were over-hydrated and those of S were dehydrated. The absolute values of embryo's weight, growth rate, oxygen consumption and the weight of the chorioallantoic membrane were similar between S and L during the first half of incubation, and greater in L in the second half. Incubation in hypoxia reduced growth rate in both sets and maintained the difference in growth trajectories between S and L. The energetic cost of growth and tissue maintenance did not differ significantly. It is concluded that,10+9 among chicken eggs of different sizes, 1) the growth rate of the embryo relates to the size of its egg, probably genetically and because of differences in water content, 2) eggshell conductance contributes, but incubation time does not, to the requirements for water loss. Therefore, the egg water balance during incubation may be the physiological constraint that limits the maximal variability in egg size compatible with embryonic survival.