Abstract

An experiment was conducted at the tissue culture laboratory of MGM college ofAgricultural Biotechnology, Aurangabad (M.S.) during 2013-14 to evaluate the effects of different concentration levels of auxins viz., NAA and IAA on growth and quality of cotton (Gossipium hirsutum L.) ovules under in–vitro conditions. The experiment was laid out in Completely Randomized Design, 7 different concentration levels of auxins IAA and NAA. Auxins were tried at the levels of 0, 5, 10 and 15 μM conc. of each in culture of 2 DPAflowers of G. hirsutum Lusing Beasley and Ting (BT) medium. Cultures were maintained for 21 days and fibre length, fibre weight, ovule length and ovule weight were recorded. Different concentrations of auxins significantly influenced development of cotton ovules under in-vitro conditions. NAAat concentration of 15 μM was found significantlysuperior over rest ofthelevels of IAAand NAA in case of fibre length, ovule length and ovule weight whereas IAA concentration of 15 μM was found significantly superior over rest of other concentrations of IAA and NAA for increasing fibre weight.

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