An efficient in vitro callogenesis from various explants of Passiflora vitifolia kunth: An important medicinal and ornamental plant

Abstract

An efficient In vitro protocol was established for callogenesis from various plant parts (Leaf, stem, flower bud and tendril) of Passiflora vitifolia Kunth. On Murashige and Skoog (MS) basal medium supplemented with various concentration of auxins (NAA, IAA, 2,4-D) and cytokinins (BAP and Kn ). Different concentrations of auxins (0.5 to 4 mg/L), IAA (0.5 to 2mg/L), NAA (0.5 to 2 mg/l) and cytokinin BAP (0.5 to 2 mg/L), Kn (0.5 to 2 mg/L) were assessed for callus induction. Growth regulators when supplemented individually on MS medium produced hard white callus in stem and tendril explant, soft friable callus in leaf and flower bud explants. The callogenic response was more in leaf, stem and flower explant on MS media supplemented with NAA (1.5mg/L) and the callogenic response was more in tendril explants on MS media supplemented with 2,4-D (1mg/L).