Growth, Leaf Morphological and Physiological Adaptability of Leaf Beet (Beta vulgaris var. cicla) to Salt Stress: A Soil Culture Experiment

Abstract

Soil salinization is a worldwide problem affecting agriculture and the environment. Utilizing saline land by screening and cultivating salt-tolerant crops is an economical and ecological solution. Leaf beet (Beta vulgaris var. cicla) is an important vegetable resource and has certain salt tolerance, but what concentration it can tolerate still remains to be verified. Hence, this work explored the adaptability of leaf beet to salt stress. A soil culture experiment was conducted. By adding NaCl to the soil, the concentrations of 0% (S0), 0.3% (S3), 0.5% (S5) and 0.7% (S7) NaCl salinity were set to analyze the changes of leaf beet growth (plant height, leaf number and biomass); leaf morphology (leaf area, shape, leaf mass per area (LMA) and tissue density); and leaf physiology (chlorophyll content, leaf relative water content (LRWC), water content at saturation (WCS), water saturation deficit (WSD) and succulence). The results showed that 0.3% NaCl salinity increased LMA and decreased biomass, while the rest were almost the same as the control (S0). Under 0.5% and 0.7% NaCl salinity, LRWC decreased significantly, while WCS and WSD increased significantly. Under such salt stress, the leaf beet adapted by reducing plant height, delaying the engendering of new leaves, decreasing leaf area, changing leaf shape, maintaining high chlorophyll content, and increasing LMA, tissue density and succulence. With these strategies, the leaf beets survived under salt stress up to 0.7% NaCl salinity with a certain biomass, providing a meaningful exploration for the multi-purpose planting and application of leaf beets on saline soils.