Abstract
The growth kinetics of a mouse-mouse hybridoma cell, 4C10B6, in a high density culture were compared with those in a low density culture. Lower glucose and glutamine consumption rates were observed in the high density culture, resulting in lower lactate and ammonium production rates. On the other hand, the specific oxygen consumption rate did not decrease in the high density culture. Higher growth yields against glucose and glutamine were obtained in the high density culture than in the low density one.
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