Growth improvement of Lolium multiflorum Lam. induced by seed inoculation with fungus suspension of Xerocomus badius and Serendipita indica

Binghua Liu,Wenxin Zhang,Bingyao Ma,Fangchun Liu,Lin Peng,Hailin Ma,Xinghong Liu

doi:10.1186/s13568-019-0865-7

Binghua Liu, Wenxin Zhang + Show 5 more

Open Access

https://doi.org/10.1186/s13568-019-0865-7

Copy DOI

Journal: AMB Express	Publication Date: Sep 12, 2019
Citations: 11	License type: open-access

Affiliation: Shandong Academy of Forestry

Abstract

In this study, a pot experiment was carried out in greenhouse to investigate the potentials of Xerocomus badius and Serendipita indica to penetrate and colonize roots of ryegrass (Lolium multiflorum Lam.) and to induce beneficial effects on seed germination and seedling growth. The results showed that X. badius and S. indica successfully colonized in the root system of L. multiflorum seedlings and the root colonization rate was 72.65% and 88.42%, respectively. By microscopy, the hyphae, chlamydospores and spores produced by S. indica were observed in roots cortex of L. multiflorum seedlings. In comparison with the non-inoculated seedlings, seedlings inoculated with X. badius and S. indica showed significant increase in growth parameters with plant height, basal diameter, biomass accumulation, relative growth rate, leaf relative water content and chlorophyll content. Also, we found that seedlings inoculated with S. indica exhibited a greater growth-promotion as compared with X. badius-inoculated seedlings. No significant influence of the two fungus application has been observed with respect to seed germination. It suggested that well establishments of mutualistic symbiosis between L. multiflorum and X. badius or S. indica were not so essential to seed germination but contributed highly to the survival and growth of the seedlings.

Highlights

Plant growth-promoting fungi (PGPF) are heterogeneous group of nonpathogenic fungi that live freely in the root surface or the interior of the root itself or the rhizosphere and mediate improvements in seed germination, seedling vigor, plant growth, flowering and productivity of a wide range of host plants (Hossain et al 2017)
Previous researches showed that ryegrass (Lolium spp.) support arbuscular mycorrhizal fungi and endophytes and establish well mutualistic symbiosis with them (Gundel et al 2011; Ponce et al 2009). In view of these findings, we propose that L. multiflorum is expected to establish mutualistic symbiosis with X. badius and S. indica, that could be beneficial to the improvement of seed germination and seedling establishment
The general objective of this research was (1) to verify the mutualistic symbiosis between L. multiflorum and X. badius or S. indica driven by seeds inoculation, and (2) to evaluate the effect of seed priming with fungus suspension on root colonization, seed germination, morphological and physiological responses of L. multiflorum

Summary

Introduction

Plant growth-promoting fungi (PGPF) are heterogeneous group of nonpathogenic fungi that live freely in the root surface or the interior of the root itself or the rhizosphere and mediate improvements in seed germination, seedling vigor, plant growth, flowering and productivity of a wide range of host plants (Hossain et al 2017). Seed bio-priming with mycorrhizal fungi has been proved to be beneficial to seed germination, seedling survival and timely seedling establishment (Prasad et al 2016; Varma et al 2017), detrimental effects of various fungi colonization on these parameters have been observed (Owen et al 2015). Application of beneficial PGPF in agricultural practices promises to be a fundamental tool to achieve sustainability in crop production and feed a constantly growing global population (Hock 2012)

Objectives

Methods

Results

Conclusion