Abstract
Growth and podophyllotoxin production of callus tissues were examined under various culture conditions. IAA and/or NAA in the presence of kinetin and casamino acids promoted callus growth, while 2,4-dichlorophenoxyacetic acid (2,4-D) yielded maximum podophyllotoxin. With respect to kinetin, the optimum concentration for callus growth and podophyllotoxin formation was 0.2 ppm, with higher concentrations inhibiting podophyllotoxin biosynthesis. The accumulation of podophyllotoxin was primarily restricted to the rapid growth phase of callus tissues and was strongly affected by the light quality and intensity. The results also indicated that the contents of podophyllotoxin and other lignanes were dependent on the conditions used for callus initiation as well as on the plant part frrm which they were derived.
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