Groundwater microbial communities and their connection to hydrochemical environment in Golmud, Northwest China

Abstract

Groundwater microbial community normally co-varies with the associated geochemical transect in some hydrogeological sections along flowpath. However, in hydrogeological section with similar geochemical transect (e.g., salinity, ion compositions) how microbial community in groundwater varies are poorly understood. In this study, groundwater samples were collected at six boreholes vertically and horizontally along a generalized groundwater flowpath in the Golmud area, Qaidam Basin, northwest China. High-throughput sequencing and multivariate statistical analysis were applied to explore the underlying relationships between microbial community structure and hydrogeochemical environment. The result showed that microbial communities changed considerably at both horizontal and vertical scales, although the groundwater samples were of relatively stable ionic compositions and hydrochemical types. The dominant bacterial phyla in groundwater varied from Alphaproteobacteria, Betaproteobacteria and Flavobacteriia in ‘phreatic and phreatic-like’ groundwater in the recharge area to Gammaproteobacteria in the confined groundwater in the lacustrine plain. At both vertical and horizontal scale, Gammaproteobacteria increased while Alpha- and Betaproteobacteria decreased as the function of distance. Genera Roseateles, Aquabacterium, Sphingomonas, Acinetobacter, Acidovorax and Flavobacterium presented in phreatic groundwater, while Pseudomonas, Hydrogenophaga and Perlucidibaca presented in confined groundwater. Spatial distribution of microbial community was highly affected by the pH (for ‘phreatic and phreatic-like’ groundwater) and ORP (for confined groundwater) of groundwater that had similar salinity or ion compositions. This research extends our knowledge about microbial communities' variation along groundwater flowpath in studied area and similar arid or semi-arid areas.