Grifola frondosa Extract (GFE) Lowers the Lymphatic Absorption of Cholesterol in Rats

Abstract

Recently, we have shown that GFE supplementation improves hyperlipidemic conditions in high fructose fed rats. In this study, we evaluated the inhibitory effect of GFE on the intestinal absorption of cholesterol in rats. Male rats with lymph cannulae were infused at 3.0 mL/h for 8 h via a duodenal catheter with a lipid emulsion containing 1.0 μCi cholesterol labeled with 14C (14C‐ cholesterol), 452 mol triolein, 75.4 mol retinol, 20.7 mol cholesterol, and 3.1 mol α‐tocopherol, and 396.0 mol Na‐taurocholate with or without GFE (100.0 mg/rat/8h) in 24 mL PBS buffer (pH, 6.4). Lymph was collected hourly for 8 h from lymph duct cannulated rats. Results showed that GFE significantly lowered the lymphatic absorption of 14C‐cholesterol (26.6 ± 4.2% dose in GFE‐infused rats vs. 35.6 ± 2.7% dose in control rats), with a significantly higher amount of 14C‐radioactivity remained in the small intestinal lumen and cecum in rats infused with GFE. The findings provide direct evidence that GFE has an inhibitory effect on the intestinal absorption of cholesterol by enhancing the luminal transport of cholesterol into the cecum. The mechanism(s) by which GFE affects the intestinal absorption of cholesterol warrants further studies.Support or Funding InformationSupported by Business for Cooperative R&D between Industry, Academy, and Research Institute funded Korea Small and Medium Business Administration in 2014.