Greenhouse evaluation of branching, leaf yield and biochemical compositions of Stevia rebaudiana Bertoni to decapitation and foliar application of abscisic acid and fluridone.

Abstract

Stevia is a herbaceous plant containing non-sugar sweeteners that could be regarded as a successor to sugar for diabetics. However, there are some problems with the cultivation of stevia, especially in the greenhouse, including unbranching and low sweetening agents. To overcome this issue, an experiment was designed to increase not only the branching and leaf production but also the sweetness. Therefore, a novel strategy using abscisic acid (0, 50, 100, 150 μM), its inhibitor, i.e. fluridone (0, 50, 100, 150 μM) and decapitation of plant apical meristems was applied. Results showed that when stevia was decapitated, dormant buds responded to the application of abscisic acid and fluridone. Under these conditions, axillary buds were developed to branches. As well leaf, total dry weight, soluble sugars and steviol glycosides (SGs) were significantly increased. In addition, the interaction of abscisic acid (50 and 100 μM) and fluridone (50 μM) had the highest positive effects on plant growth and steviol glycosides production rather than their sole applications. Results also indicated that decapitation removed a terminal dominance over a limited period of time and the terminal dominance was re-established with the growth of the terminal branches, whereas the influence of 50 µM fluridone on stevia was long term and the number of shoots was greater. Since stevia is a costly sweetener, the results of this study could be used in greenhouses, where the cultivation of stevia seems to be reasonable in terms of economic aspects.