Effects of Abscisic Acid on Drought Responses of Kentucky Bluegrass

Abstract

Abscisic acid (ABA) is an important hormone regulating plant response to drought stress. The objective of this study was to investigate effects of exogenous ABA application on turf performance and physiological activities of kentucky bluegrass (Poa pratensis L.) in response to drought stress. Plants of two kentucky bluegrass cultivars, `Brilliant' (drought susceptible) and `Midnight' (drought tolerant), were treated with ABA (100 μm) or water by foliar application and then grown under drought stress (no irrigation) or well-watered (irrigation on alternate days) conditions in a growth chamber. The two cultivars responded similarly to ABA application under both watering regimes. Foliar application of ABA had no effects on turf quality or physiological parameters under well-watered conditions. ABA application, however, helped maintain higher turf quality and delayed the quality decline during drought stress, compared to the untreated control. ABA-treated plants exposed to drought stress had higher cell membrane stability, as indicated by less electrolyte leakage of leaves, and higher photochemical efficiency, expressed as Fv/Fm, compared to untreated plants. Leaf water potential was not significantly affected, whereas leaf turgor pressure increased with ABA application after 9 and 12 d of drought. Osmotic adjustment increased with ABA application, and was sustained for a longer period of drought in `Midnight' than in `Brilliant'. The results suggested that exogenous ABA application improved turf performance during drought in both drought-sensitive and tolerant cultivars of kentucky bluegrass. This positive effect of ABA could be related to increased osmotic adjustment, cell turgor maintenance, and reduced damage to cell membranes and the photosynthetic system.