Green synthesis of zinc oxide nanoflowers using Hypericum triquetrifolium extract: characterization, antibacterial activity and cytotoxicity against lung cancer A549 cells

Abstract

Zinc oxide nanoparticles have attracted significant interest in recent years due to their unique multifunctional chemical and physical properties along with their biological activities. This study demonstrated for the first time the biogenetic synthesis of zinc oxide nanoparticles by utilization of the methanolic extract of Hypericum triquetrifolium (HT). The obtained nanoparticles (HT‐ZnO) were characterized by ultraviolet–visible spectroscopy (UV–Vis), Fourier‐transform infrared spectroscopy (FTIR), X‐ray diffraction (XRD), dynamic light scattering (DLS), scanning electron microscopy (SEM), energy dispersive X‐ray spectroscopy (EDX), and transmission electron microscopy (TEM). The shape of the resulted nanoparticles is fusiform nanoflowers with an average hydrodynamic size of 275.46 ± 0.20 nm and a zeta potential of −8.23 ± 0.26 mV. SEM micrographs revealed that HT‐ZnO nanoflowers have a multi‐process structure in which one of the processes is large and the others have similar smaller dimensions. The synthesized nanoflowers have an average length of 312.28 ± 78.93 nm and the tip of its processes has a width of 48.69 ± 9.71 nm. The antimicrobial activity of HT‐ZnO nanoflowers was performed using microbroth dilution format. It showed a bactericidal mode of action against Gram‐positive Staphylococcus aureus and Enterococcus faecalis with MIC/MBC values of 20 μg/mL and 5 μg/mL, respectively. MTT assay had revealed that HT‐ZnO nanoflowers caused a dose‐dependent decline in the viability of A549 adenocarcinomic human alveolar basal epithelial cells with an IC50 value of 20.45 μg/mL. The effect of HT‐ZnO nanoflowers on the migration and colony formation abilities against the same cells was evaluated as well. In conclusion, zinc oxide nanoflowers were successfully synthesized using methanolic extract of H. triquetrifolium. The resulting particles showed a bactericidal effect against Gram‐positiveS. aureus and E. faecalis and a cytotoxic activity against A549 cells.