Camellia sinensis (Green Tea) Mediated Synthesis of Zinc Oxide Nanoparticles and Detect its Antibacterial Activity against Escherichia coli, Staphylococcus aureus and Acinetobacter baumannii

Israa Ali Zaidan Al-Ogaidi

doi:10.24126/jobrc.2017.11.1.497

Abstract

In the current study, synthesis and characterization of Zinc oxide nanoparticles (ZnONPs) and its application as anti-pathogenic bacteria were investigated. ZnO which has been prepared by using aqueous of green tea leaves extract (Camellia sinensis) as a reducing agent. The wavelength range was measured by Ultraviolet–visible spectroscopy (UV-Vis) for monitoring the formation of the nanoparticles, which showed sharp peak at 360 nm. The average size and shape of the nanoparticles were detected by using Atomic Force Microscopy (AFM) which was 88 nm with spherical shape. Fourier transform–infrared (FTIR). FT-IR spectra was documented for the ZnO nanoparticles synthesized by green tea extract to detect the biomolecules involved in the synthesis process. The antibacterial activity of crystal Zinc Oxide (ZnO) nanoparticles was explored against pathogenic bacteria that included Escherichia coli, Staphylococcus aureus, and Acinetobacter baumannii. The antibacterial test was conducted in solid media using different concentrations of ZnO and disk diffusion method, 100 µg/ml presented the best antibacterial activity, and further studies on the damage of bacterial genomic DNA of Escherichia coli and Acinetobacter baumannii were carried out using gel electrophoresis exposed the DNA fragment bands, this activity may be caused by the interactions between the surface charge of cell and nanoparticles. Reactive oxygen species (ROS) properties of the particles might disturb cell wall and great antimicrobial action

Highlights

This study aims to use nanomaterials to achieve antibacterial activity against MDRs isolates through testing the activity of Zinc Oxide (ZnO) against MDRs isolates and test its ability to damage DNA of Escherichia coli and Acinetobacter baumannii
The Fourier transform–infrared (FTIR) studies obviously indicated the capping and reduction biomolecules being in the leave extract of green tea, the presence of high amount of biomolecules was responsible for the reduction process and the amino acids and amide bonds in protein were worked as stabilizer agents of the ZnO nanoparticles
The antibacterial tests demonstrated that 100μg/ml was the best antibacterial activity and the integrity of bacterial DNA was affected after treated with Zinc oxide nanoparticles (ZnONPs)

Summary

Introduction

‫هدف البحث الى دراسة تأثير دقائق اوكسد الزنك النانوية المحضرة بطريقة المستخلص المائي لاوراق الشاي الاخضىر كمضاد بكتيري‬ .‫ نانوميتر لدقائق اوكسيد الزنك النانوية‬360 ‫وصنفت هذه الدقائق باستخدام جهاز المطياف الضوئي والذي اظهر قمة امتصاص بحدود‬ ‫ وقد تم فحص التركيب الكيميائي‬.‫ نانوميتر وذات شكل كروي‬88 ‫وقد اظهرت نتائج القوة الذرية ان معدل حجم الدقائق النانوية بمقدار‬ ‫الدقائق النانوية باستخدام مطياف الاشعة تحت الحمراء اذ اظهر وجود المركبات الحيوية على سطح دقائق الزنك النانوية أختبرت فعالية‬ Staphylococcus aureus Acentobacter baumannii, ‫دقائق اوكسيد الزنك المصنعة كمضادات لثلاثة انواع بكتيرية هي‬ ‫ باعتماد قياس اقطار‬،‫مل اعطى افضل نتيجة تثبيط‬/ ‫ ميكىوغرام‬100 ‫في الوسط الصلب اظهرت النتائج ان تركيز‬Escherichia coli ‫ فضلا عن ذلك درس تاثير الدقائق النانوية على المادة الورثية من خلال الترحيل‬.‫التثبيط لمراقبة تأثير دقائق اوكسيد الزنك النانوية‬ ‫ او ان‬.‫الكهربائي وملاحظة تقطيع المادة الوراثية والتي من الممكن ان تعود الى التداخل مابين السطح المشحون للخلية والدقائق النانوية‬. The rapid spread of these isolates and the dangerous infections caused by them require the urge to find the replacement for the treatment of these MDR isolates derived the medical community to use them as novel antimicrobial agents. Many approaches are engaged for the synthesis of silver nanoparticles including reduction silver nitrate in solutions; microwave assisted, laser ablation, and biological or green synthesis methods [6, 8] The latter is the most favorite ecofriendly fast method for synthesizing nanoparticles [9]. The current study differs from previous reports by using the green method instead of chemical method to synthesis ZnONPs. This study aims to use nanomaterials to achieve antibacterial activity against MDRs isolates through testing the activity of ZnO against MDRs isolates and test its ability to damage DNA of Escherichia coli and Acinetobacter baumannii

Objectives

Methods

Conclusion