Green Synthesis of Well Dispersed Nanoparticles using Leaf Extract of Medicinally useful Adhatoda Vasica Nees

Abstract

Abstract: Development of reliable method for the green synthesis of gold nanoparticles (AuNPs) using medicinally valued Adhatoda vasica Nees has been studied here. The color change and the Surface Plasmon Resonance (SPR) confirmed the formation of AuNPs. The biosynthesized AuNPs were characterized using UV-visible Spectroscopy (UV-vis), Fourier Transform Infrared Spectroscopy (FT-IR), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDAX) and Transmission Electron Microscopy (TEM) analysis. The nanoparticles synthesized were predominantly monodisperse, stable spherical in nature with well-defined dimensions of size ranging from 22 to 47 nm. The crystalline nature of the synthesized particles was also evident by the X-ray diffraction analysis. Keywords: Adhatoda vasica, Gold nanoparticles, Biosynthesis, Electron Microscopy, Diffraction. INTRODUCTION Nanobiotechnology combines biological principles with physical and chemical procedures to generate nano-sized particles with well defined functions. Particles of interestingly small size make the field of drug delivery more interesting and effective. Synthesizing gold particles (AuNPs) with medicinal applications is the recent trend in the field of nanobiotechnology. The surface availability of nanoparticles for binding/reactivity of other species on them is an important function as it is synthesized in different structures like nanorods, spheres, prims and hexagons. The controlled growth of AuNPs of different morphologies and the various chemical mechanisms involved in the anisotropic growth were studied using different chemical procedures [1]. The low toxicity effects of green synthesized AuNPs on biological systems made researchers to synthesize it by biological method rather by chemical means. Extensive studies were done on AuNPs and its binding affinity towards nucleic acids and proteins in biological systems [2]. AuNPs have been synthesized from various sources like plants [3], microbes [4], seaweeds [5] and microalgae [6]”. Sastry