Green Synthesis of Silver Nitrate Nanoparticles using L. of Pyracantha crenulata and its Anticancer Activity on Liver Cancer

Abstract

The next-generation of anti-cancer medications have a lot of potential, thanks to the biocompatible silver nanoparticles (AgNPs). Here, AgNPs were produced utilizing Pyracantha crenulata leaves in a biogenic manner. Several spectroscopic methods were used to characterize and verify the produced AgNPs. The peak of the AgNP’s Surface Plasmon Resonance (SPR) at 455 nm was discovered by UV spectroscopy research. Transmission electron microscope (TEM) examination was used to determine the precise size and morphology of AgNPs, which were discovered to be 60 nm in size and spherical, triangular, and hexagonal in form. X-ray Diffraction (XRD) spectrum data supported AgNPs crystallinity. The existence of elemental Ag was confirmed by (EDX) spectra, which displayed a clear, strong signal at 3.0 KeV. Particle size analysis was used to characterize the produced Ag nanoparticles. In order to characterize the silver nanoparticles, a (DLS) zetasizer particle size analysis was performed. According to a DLS investigation, the biosynthesized silver nanoparticles are 1340.8 nm in size and have a polydispersity index (PDI) of 0.6%. Silver nanoparticle stability was assessed using a zeta potentiometer. The nanoparticles generated had a zeta potential of -18.7 mV. The leaf extract's functional groups and the AgNPs that were produced, were identified using the analytical technique, FTIR. Examining AgNPs anti-cancer efficacy against liver (hep-2) cancer revealed its strong anti-cancer potential. Our findings suggest that generated AgNPs greatly inhibit the growth of liver cancer cell (hep-2) as measured by the MTT test.