Abstract
The green route of metal nanoparticles synthesis has received significant attention in recent years due to it's cost-effective, non-toxic and eco-friendly nature in comparison to other physical and chemical methods. This study reports on the synthesis of silver nanoparticles (Ag-NPs) from bio-reduction of 1mM aqueous silver nitrate (AgNO3) by extracts prepared from three different plants namely, Brassica oleracea L. var. italica Plenck (Broccoli), Capsicum annuum L. (Chili) and Parthenium hysterophorus L. (Carrot grass). The synthesized Ag-NPs were characterized using UV- visible spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Ag-NPs synthesized showed the surface plasmon resonance with the appearance of absorption peaks around the range of 410-430 nm. The possible biomolecules involved in the reduction and the stabilization of synthesized Ag-NPs were found to be alcoholic, phenolic, amine and carbonyl groups. SEM study revealed that Ag-NPs were spherical in shape with varied size about 10-40 nm. Besides, the analysis of antioxidant and antibacterial activities of Ag-NPs was carried out. The Ag-NPs synthesized using B. oleracea extract showed the higher antioxidant activity than Ag-NPs synthesized from both C. annuum & P. hysterophorus extracts. Ag-NPs exhibited good antibacterial activity against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. The higher antibacterial activity was shown by Ag-NPs synthesized from P. hysterophorus extract in comparison to Ag-NPs synthesized from both C. annuum & B. oleracea extracts. Hence, it can be concluded that Ag-NPs synthesized following the green route could be the source for potential antioxidant and antibacterial agents.
 Int. J. Appl. Sci. Biotechnol. Vol 6(4): 294-301
Highlights
Nanotechnology is emerging as a field of interest nowadays due to its applications in various sectors such as energy, drug delivery, medicine, electronic, optics etc. having huge potential to contribute to society
The synthesis of metal nanoparticles following green route has gained a lot of attention in recent years due to its ecofriendly and non-toxic nature
The synthesis of silver nanoparticles (AgNPs) from bio-reduction of silver ions using the extracts of P. hysterophorous, C. annuum and B. oleracea was carried out
Summary
Nanotechnology is emerging as a field of interest nowadays due to its applications in various sectors such as energy, drug delivery, medicine, electronic, optics etc. having huge potential to contribute to society. Having huge potential to contribute to society It is the science of manipulating matter at nanoscale level (1-100 nm) to create new materials having improved physical and chemical properties. Nanoparticles synthesis can be carried out by three different approaches which are physical, chemical and biological methods. Biological methods involve the synthesis of nanoparticles through the use of biological components such as plant extracts, bacteria, and fungi. The green route of nanoparticles synthesis could be the best alternative for physical and chemical methods. Parthenium hysterophorus contains various compounds having medicinal values such as saponin, histamine, glucosides, and triterpenes. It was used as a folk remedy against ulcerated sores, facial neuralgia, anemia and fever (Kushwaha and Maurya, 2012). We have reported on the synthesis of Ag-NPs, characterization of synthesized Ag-NPs and evaluation of their antioxidant & antibacterial activities
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