Green synthesis of silver nanoparticles using cow urine: Antimicrobial and blood biocompatibility studies

Abstract

In this research, new green synthesized cow urine silver nanoparticles (CoUSiN particles) were accomplished by using cow urine as a reducing and capping agent. The cow urine silver nanoparticles have been marked by UV–Visible spectroscopy, X-ray diffraction, Fourier Transformer Infrared spectroscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy. The size of the CoUSiN particles had probed by using a dynamic light scattering method and was determined to be 47.8 ​nm. Further, CoUSiN particles evidenced biocidal venture against Escherichia coli and methicillin-resistant Staphylococcus aureus through cell wall destruction and membrane poring. The SEM established the absolute biocidal sheath destabilization of Escherichia coli and whereas the cyclic voltammetry (CV) bolstered the membrane poring mechanism on methicillin-resistant Staphylococcus aureus . Further, the blood congenial nature of CoUSiN particles fortified that it can be a promising candidate to heal epizootic diseases. The inquiry infers that the CoUSiN particles incorporated adopting a simple, inexpensive, eco-friendly modus using cow urine as a reducing agent. “ Death of bacteria by CoUSiN interactions”. • CoUSiN particles is synthesized using cow urine. • The antimicrobial properties of CoUSiN particles using Gram-positive and Gram-negative bacteria such as E.coli and MRSA were evaluated. • The study also establishes blood compatibility of CoUSIN particles.