Green synthesis of silver nanoparticles using aqueous leaf extract of Guettarda Speciosa and its antimicrobial and anti-oxidative properties

Abstract

The current work details the green synthesis of silver nanoparticles (AgNPs) by preferably choosing Guettarda Speciosa (G.Speciosa) leaf extract as an effective reducing agent. The formation of AgNps (silver nanoparticles) was confirmed visually with the change in the colour illuminated by the nanoparticles in the solution. Further, it was examined by UV-–Vis spectroscopy within the wavelength of 200–800 nm, FT-IR, DLS, FE-SEM, EDAX and XRD. The UV–Vis spectrum shows the presence of the AgNPs at 428 nm. FT-IR studies reveal the persistence of various functional groups and their corresponding bonds present in the material. DLS predicts the size of AgNPs was in the range of 6.5 nm and 160 nm and it was confirmed with the FE-SEM analysis accurately as 30 nm. Hence the work promptly delivers the biosynthesized AgNps as an antimicrobial agent hostile to contagious microorganisms and also, as an aggressive anti-oxidant. Moreover, AgNps were tested with two gram-positive (Staphylococcus Aures (MTCC 25,923), Basillus Subtilis (MTCC 2451)) and two gram-negative (Escherichia coli(MTCC 25,922), Streptococcus Aureus (MTCC 273)) microbes and its active response could ensure it as a possible antimicrobial source and its related medical applications.