Abstract
Background: Considering the high cost of nano-particles chemical synthesis and bacteria’s high resistance against antibiotics, investigating silver particles biosynthesis and their effect on clinical and standard strains of different bacteria is very important. Objective: This study investigates the feasibility of green synthesis of silver chloride nanoparticles by labiosa (Bunge) Adylov, Kamelin & Makhm Phlomoides (Eremostachys labiosa Bunge) herbal extract and Phlomoides binaludensis Salmaki & Joharchi (Binaloud Cistanche tubulosa) plant. Methods: Scanning Electron Microscope (SEM), X-Ray Diffraction (XRD), UV-Visible Spectroscopy, and Energy-Dispersive X-ray Spectroscopy (EDS) are used for nano-particles characterization. The antibacterial property of extracts and synthesized nano-particles was evaluated against Staphylococcus aureus, Bacillus cereus and Escherichia coli by agar disk diffusion and well diffusion, respectively. As antioxidants existing in plants are expected to act as regenerators in nano-particles synthesis, the plants used were investigated considering the existence of antioxidants through two DPPH and FRAP methods. Total values of phenol and IC50 were determined by the extract of considered plants. Results: The results showed that what has been successful in nano-particles synthesis is silver chloride nano-particles synthesis, which is due to the existence of chlorinated compounds in the herbal extract. Synthesized nano-particles are spherical, and their size is in the range of 27-35 nm, and synthesized nano-particles were distributed consistently. Also nano-particles enjoy significant antibacterial activity. Conclusion: IC50 was 0.56 mg/ml and 0.96 mg/ml for the aerial organs of Eremostachys labiosa Bunge and Binaloud Cistanche tubulosa, respectively, in this study, while it was 0.38 mg/ml for BHT synthetic antioxidant. Also, iron regeneration ability reported 255.990 and 64.110 Fe ion mmol/extract gr by the extracts.
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