Green Synthesis of MnO2 NPs Using Blumea lacera Leaf Extract and its Antimicrobial Study

Abstract

Background: Green synthesis of nanoparticles has emerged as an interesting and expanding research area due to environmental friendliness, non-toxicity, cleanliness, and cost-effectiveness. Moreover, it can be performed at room pressure and temperature. Blumea lacera is described as a valuable medicinal plant in many vital systems of medicines. The study explored the eco-friendly green synthesis of MnO2 NPs using Blumea lacera leaf extract. Methods: Reduction of potassium permanganate (KMnO4) using Blumea lacera leaf extract was carried out at room temperature. The crude extract of Blumea lacera was added to metal ion reagents of specific volume and specific concentration at ambient temperature and stirred continuously using a magnetic stirrer. The aqueous leaf extract reduced and stabilized the KMnO4 into MnO2 NPs. The MnO2 NPs obtained from the solution were purified and separated by repeated centrifugation using Remi cooling centrifuge model C-24. Results: The biosynthesized MnO2 NPs characterized by UV–Vis spectroscopy showed an absorption peak at 400 nm. The XRD studies revealed the spherical shape of MnO2 NPs with an average particle diameter of 20 nm. FT-IR analysis confirmed the presence of functional groups -OH, C=O, C=C, and CH triggering the synthesis of MnO2 NPs. Vibrational mode at around 606.62 and 438.81 cm−1 supports the occurrence of the O–Mn–O bond. Conclusion: The synthesized MnO2 NPs were found to be good antibacterial and antifungal agents against bacterial strains Staphylococcus aureus, B. subtilis, Pseudomonas aeruginosa, E. coli, and fungal strains C. albicans, Aspergillus niger, and Sclerotium rolfsii.