Green Synthesis of Copper Nanoparticles Using Leaves and Flowers Extract of Cassia auriculata L. and Study of It’s Antimicrobial Activity.

Abstract

Abstract: Evolution of biologically inspired experimental processes for the synthesis of nanoparticles is developed into an important branch of nanotechnology. The leaf and flower extract of Cassia auriculata L. ( Caesalpiniacece) in the synthesis of copper nanoparticles was investigated employing UV/Ultra visible spectrophotometer, SEM/scanning electron microscopy and PXRD/Powder X-ray diffraction. Uv shows the sharp band of copper Nanoparticles is around 375nm of Cassia auriculata (fresh and dry leaf and flower) , SEM image has shown the morphological shape of the copper nanoparticles C.auriculata fresh leaf copper nanoparticles average size is 12.43nm, dry leaf is 40nm and fresh flower copper nanoparticles size is 57.87nm, dry flower is 7.14nm, X-ray diffraction patterns of synthesized fresh leaves mean size of copper nanoparticles calculated using the DebyeScherrer’s equation was found to be 12.43nm,mean size of dry leaves is 40nm,mean size of fresh flower is 57.87nm and mean size of dry flower is 7.14nm respectively.The results of antimicrobial activity of the C. auriculata green synthesized CuNPs against the Gram- negative bacterial strains (E. coli and S.typhi) and the Gram-positive bacterial strains (S.aureus and B.subtilis), Fungi (Aspergillus niger, Fusarium oxysporum, Penicillium atramentosum, Aspergillus flavus) under the well diffusion method had a considerable antimicrobial activities increased with higher concentration. The fresh leaves CuNPs shows more effective against S.aureus, dry leaves CuNPs against E. coli, fresh flowers CuNPs against S. aureus and dry flowers CuNPs shows more effective against B. subtilis. In case of fungi both fresh and dry leaves and flowers CuNPs shows more effective against F.oxysporum. C. auriculata is a medicinal shrub or tree which is native to the India, Myanmar and Sri Lanka. This plant reported for its uses in the Ayurvedic and siddha system of medicine for treating various diseases mainly both the diabetes types. The antimicrobial potential of the leaf and flower extract was tested. The anti-bacterial and anti-fungal property were found to be better for the aqueous leaf and flower extract and the extract containing the metal particles.