Green Synthesis of Copper Nanoparticles using Green Tea and Neem Formulation and Assessment if its Antimicrobial Effects

Abstract

Aims: Nanotechnology is the science which is about manipulating matter, atom by atom and is associated with particles smaller than 100 nm in size. Copper nanoparticles are used mainly due to its surplus amount, low cost, easy availability and biocompatible property. Green synthesis of copper nanoparticles is very simple, economical and eco-friendly method that does not involve any toxic chemicals. The aim of our study is green synthesis of copper nanoparticles using green tea and neem formulation and assessment of its antimicrobial effects.Place and Duration of Study: Sample: Department of Nanotechnology, Saveetha dental college and hospitals, between December 2020 and January 2021Methodology: 20 mM of copper sulphate solution is mixed with 40mL of plant extract and 60 mL of distilled water was added and made it into 100 ml solution. Once the copper nanoparticles are synthesized the solution is characterized using UV- vis-spectroscopy and was scanned in double beam UV-vis- spectrophotometer from 300 nm to 700nm wavelength. The antimicrobial property of copper nanoparticle is evaluated by agar well diffusion method.Results: The colour change from green to brown and peak observed in UV-vis- spectrophotometer was associated with the synthesis of copper nanoparticles. Copper nanoparticle from green tea and tea extract has good antimicrobial activity against S.mutans, C.albicans, E.faecalis, S, aureus.Conclusion: Copper nanoparticles can be efficiently synthesised from green and neem formulation.hese copper nanoparticles showed good antibacterial properties and are effective against oral pathogens