Evaluation of cytotoxic and antioxidant properties of copper nanoparticles using green tea and neem formulation

Abstract

Introduction: Copper nanoparticles are of great interest and have applications in various fields due to their antibacterial, antioxidant, cytotoxicity and anticancer properties. Green synthesis of nanoparticle is currently a new approach which is a non toxic,economical,eco friendly approach. Our study aim was to assess the antioxidant and cytotoxic effects of green synthesized copper nanoparticles from green tea and neem formulation. Materials and methodology:20mM of copper sulphate solution is mixed with 40mL of plant extract and 60 mL of distilled water was added and made it into 100 ml solution.The copper nanoparticles derived using green synthesis was assessed for antibiotic activity by free radical scavenging assay using 1,1-diphenyl-2-picrylhydrazyl(DPPH). Cytotoxicity of copper nanoparticles was evaluated using Brine shrimp assay at 5,10,20,40 and 80µL concentrations. Results: The change of colour from green to brown and the peak observed in UV-vis- spectrophotometer indicated the synthesis of copper nanoparticles. The antioxidant property was visually observed by the colour change from green to brown indicating that DPPH has undergone a reduction reaction exhibiting scavenging activity. The cytotoxic effects of green synthesised copper nanoparticles from green tea and neem extract showed that there is a concentration dependent increase in the cytotoxic property of copper nanoparticles.