Green Synthesis of Chitosan Nano Particles at Different Temperature for Bio-Medical Applications

Abstract

Chitosan is a semi biopolymer obtained from natural biopolymer Chitin by depolymerisation and de-acetylation. It is bio-degradable, non-toxic and an ideal candidate for pharmaceutical uses. But major bottle-neck of biomedical application of chitosan is that it is insoluble in water therefore we have reported here preparation of ionically cross linked chitosan nano material using tartarate ion as ionic cross linker by greener hydrothermal technique at different temperature and pressure to regulate particle sizes. To the best of our knowledge this is the first time we are reporting hydrothermal synthesis of chitosan nanoparticles at high pressure to regulate particle sizes. Hydro- dynamic particle sizes have been determined by DLS measurement. We have also studied fluorescence behaviour of these chitosan nanoparticles and studied their anti-bacterial activities by Alamar assay on E Coli and S Aureus.