Green synthesis and in-vitro screening of 3-((4-(arylideneamino)phenyl) imino)indolin-2-one derivatives antimycobacterial agents

Abstract

In this research article, we attempted to synthesize some new isatin derivatives, 3-((4-(arylideneamino)phenyl)imino)indolin-2-ones (2a-2g) by Schiff\'s base reaction under microwave technique. The structural characterizations of synthesized compounds were done by using different analytical techniques such as IR, 1HNMR, mass spectrometry, and elemental analysis. We aim to develop new compounds with improved antimycobacterial activities. These title compounds were screened for their antimycobacterial activity against the Mycobacterium tuberculosis H37Rv strain by using the microplate Alamar blue dye assay (MABA) method. All title compounds showed significant antimycobacterial activity. Compound 2g was found most potent compound with a MIC value of 6.25 µg/mL among all tested compounds when compared with standard drugs streptomycin (6.25µg/mL) and isoniazid (6.25 µg/mL).