Abstract
Background: Zinc oxide Nanoparticles can be prepared by various chemical, physical, and biological methodologies. But biological approach is easier than the other methods and also eco-friendly. 
 Purpose of study: Plant-mediated amalgamation of Zinc oxide nanoparticles is promising contrast to traditional method of physical and chemical synthesis. 
 Method: In the current research work, Zinc oxide nanoparticles are synthesized by using Leaf extracts of Manilkara Zapota plant. Green synthesis was done utilizing the aqueous solution of Manilkara Zapota leaf extract as reducing agent and Zinc acetate salts along with Sodium hydroxide. Morphological, Structural and Antimicrobial properties of synthesized Zinc oxide nanoparticles were characterized using Scanning electron microscope, Fourier transform infrared analysis, Differential scanning calorimetry and Disc diffusion method. '
 Results: Biologically synthesized nanoparticles of Manilkara Zapota leaf extract revealed significant Anti-bacterial and Anti-fungal activities. 
 Cocnclusion: The organically incorporated nanoparticles by Manilkara Zapota can be utilized as antimicrobial agent against various pathogenic organisms.
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