Green synthesis and characterization of silver nanoparticle from ampelocissus latifolia root extract

Abstract

In the present research work, green synthesis of silver nanoparticles (AgNPs) has been demonstrated using root extract of Ampelocissus latifolia (Vitaceae family) reducing aqueous silver nitrate. The synthesized nanoparticles were characterized by different instrumental techniques. Change of color and stability studies were identified by Ultraviolet-Visible (UV-Vis) spectrometer; Surface morphology and elemental composition of AgNPs studied by Scanning electron microscope (SEM) and Energy Dispersive X-ray spectroscopy (EDAX); Selected Area Diffraction Pattern (SAED) and TEM micrographs showed spherical particles with an average size of 35-45 nm. The FCC structure and crystalline nature of prepared nanoparticles identified by the XRD technique based on the characteristic Bragg peaks of (111), (200), (220), (311) and (222). Further, prepared silver nanoparticles showed effective antibacterial activity toward gram positive and negative bacteria. This examination demonstrated that silver nanoparticles posses extensive antibacterial activity in correlation with standard antibacterial agents, and hence further investigation or clinical applications is necessary.