GREEN SYNTHESIS AND CHARACTERIZATION OF ISOLATED FLAVONOID MEDIATED COPPER NANOPARTICLES BY USING THESPESIA POPULNEA LEAF EXTRACT AND ITS EVALUATION OF ANTI-OXIDANT AND ANTI-CANCER ACTIVITY

Abstract

Objective: An Eco-friendly process of Green Synthesis of Copper Nano-particles (CuNPs) is an important aspect in the field of Nanotechnology using alternative feedstock, energy minimization, the design of less toxic and inherently safer chemicals. Methods: In this study, Copper Nano-Particles were synthesized by using isolated flavonoids to induce the reduction of Cu2+ions to CuNPs and also act as a capping and stabilizing agent. The solutions of CuSO4 and flavonoid were used as stock solutions for the preparation of CuNPs. Aqueous flavonoid(Quercetin) solution was mixed with CuSO4 solution separately. The reaction mixtures were immediately placed in a hot plate magnetic stirrer at 2000 rpm, 50-60 °C temperature and observed the change in colour of the solution from colourless to coloured solution. The synthesized CuNPs were characterized by various spectral methods for structural analysis of Nano-particles and In vitro and In vivo evaluation for anti-cancer activity. Results: The results described that the Copper Nano-particles are crystalline and amorphous with an average size of 295.4 nm and highly stable and having good hydrogen peroxide scavenging effect, reducing power and total antioxidant activity with the IC50value of 59.24μg/ml, 24.35μg/ml and16.83μg/ml respectively. The in vitro (HepG2 and MCF-7 cell lines) anti-cancer activity showing good results with IC50 values of 57.56 µg/ml for HepG2 cell line and 56.41 µg/ml for MCF-7 cell line. The in vivo anti-cancer activity also showing good results.