Abstract
ABSTRACTAlternanthera philoxeroides (Mart.), a weed, mainly from tropical origin and easily available worldwide. People used to eat it as a food mainly in South Africa. In our previous report we have thoroughly characterise several important phenolics, monoterpene and phenylpropane from methanol soluble fraction of Alternenthera leaves (fraction X) and also reported their α-glucosidase inhibitory, antimicrobial and antioxidant activities. All these isolated natural compounds are well characterised and widely studied. In our present study we try to use this beneficial fraction (named fraction X) in green synthesis of gold nanoparticles (X-GNP). We also try to explore the beneficial aspects of green synthesis in comparison with commonly used chemical synthesis method (GNP) in context with their antimicrobial activity. UV/Vis spectroscopy, DLS, Zeta potential, FT-IR, EDAX and other microscopic techniques namely: SEM, AFM were used to characterise the synthesised nanoparticles. Different important microbial strains were used to evaluate the antimicrobial activity of prepared nanoparticles. Overall the studies suggest successful synthesis of green nanoparticles (X-GNP) and also showed the improvement in antimicrobial activity of X-GNP nanoparticles.
Highlights
Nanotechnology a days become an exciting tool to design different types of particles which have a vast application in the field of medicine, diagnostic, imaging, drug discovery, tissue engineering and many more [1]
Nanotechnology is one of most widely studied topic mainly deals with synthesis and manipulation of small particles
The secondary metabolites present in the plant extracts play the major role in the green synthesis [35]
Summary
Edmonton, AB T6G 2P5, Canada +Department of Botany, Jhargram Raj College, Jhargram, Paschim Medinipur, West Bengal, India. Gold nanoparticles mainly synthesised by using well established chemical method (citrate reduction) and due to its small size, inert character it has been widely used in different drug targeting and medicinal studies [4]. To reduce the side effects several biological methods are recently used to synthesise gold nanoparticles [5]. We have used methanol soluble fraction of Alternanthera philoxeroides (Mart.) Grisb. In our previous study we have thoroughly characterised the methanol soluble fraction of A. philoxeroides leaves [21]. Kaempferol, ferulic acid, salicylic acid, syringic acid, chlorogenic acid, one monoterpene (safrole) and one phenylpropane (myrcene) in the methanol soluble fraction called fraction-X [21]. Gold nanoparticles were synthesised using fraction-X of A. philoxeroides leaves (X-GNP) in comparison to chemically synthesised nanoparticles (GNP). Synthesised nanoparticles were characterised by using spectroscopic (UV/Vis, DLS, Zeta potential, FT-IR, EDAX), microscopic (SEM, AFM) methods and antimicrobial property was evaluated against Pseudomonas aeruginosa (MTCC 10462), Escherichia coli (MTCC 1680), Micrococcus luteus (MTCC 8101), Acinetobacter lwoffii (MTCC 8288) and Bacillus subtilis (MTCC 10073)
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