Green in situ immobilisation of gold nanoparticles on bacterial nanocellulose membranes using Tannic acid and its detection of Fe3.

Abstract

Oxidative stress is one of the factors that promote melanogenesis. Trivalent iron ions play a key role in regulating the iron-catalysed oxidative stress response. A novel SERS flexible membrane sensor based on tannic acid with good sensitivity and uniformity was prepared by green in situ reduction of gold nanoparticles on bacterial cellulose membrane（BCM）with a simple and highly selective method to detect Fe3+. Under alkaline conditions, Fe3+ is adsorbed on the BCM-TA@Au NPs flexible membrane by tannic acid (TA) through chelation, thus enabling the detection of Fe3+. Furthermore, this simple detection system has a wide linear detection range and high sensitivity to effortlessly evaluate Fe3+ at concentrations up to 10-7 M. More importantly, the proposed SERS flexible substrate performed well in determining Fe3+ concentrations in B16 melanocytes, providing new insights into the factors affecting the melanin synthesis pathway and providing a potential biomarker for melanoma treatment.