Green Biosynthesis of Silver Nanoparticles Using Marine Red Algae Acanthophora specifera and its Antibacterial Activity

Abstract

Silver has been known since ancient history for its antimicrobial property. Synthesis it by eco-friendly method is a good thing due to its cost effective, stability and environmentally favorable. In this study silver nanoparticles (AgNPs) were obtained by a green synthesis method. Alcoholic extraction of Acanthophora specifera was used to reduce silver nitrate salt to AgNPs. Structure of synthesized AgNPs which observed by the dark brown color development was characterized by X-Ray diffraction (XRD), where the crystalline nature of silver nanoparticles were cubic and its size was range in 33 nm to 81 nm. Moreover, infrared spectroscopy (FT-IR) was identified. Additionally, the anti-microbial activity of AgNPs was recorded by using disk diffusion methods. AgNPs significantly reduced the growth of both gram positive Staphylococcus aureus, Bacillus subtillis, and gram negative Salmonella spp., Escherichia coli in addition to the unicellular fungus Candida albicans.