Green biosynthesis, characterization, and cytotoxic effect of magnetic iron nanoparticles using Brassica Oleracea var capitata sub var rubra (red cabbage) aqueous peel extract.

Ömer Erdoğan,Salih Paşa,Özge Çevi̇k,Gülen Melike Demirbolat,Özge Çevik

doi:10.3906/kim-2102-2

Abstract

The green method of nanoparticle synthesis, which is an environment and living-friendly method, is an updated subject that has appeared as an alternative to conventional methods such as physical and chemical synthesis. In this presented study, the green synthesis of magnetic iron oxide nanoparticles (IONPs) from iron (III) chloride by using Brassica oleracea var. capitata sub.var. rubra aqueous peel extract has been reported. The prepared IONPs were characterized with fourier-transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy (UV-VIS), zeta potential, scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). The cytotoxic effects of IONPs on MCF-7 breast cancer cell line were studied by MTT assay, and migrative effect of its were carried out by the wound healing assay. It was found that the mean particle size of IONPs was 675 ± 25 nm, and the polydispersity index was 0.265 PDI. It was also determined that these nanoparticles had an anti-proliferative impact on the MCF-7 breast cancer cell line depending on the dosage. Characterization results support the successful synthesis of nanoparticles, and the dose-dependent cytotoxic effects of nanoparticles on MCF-7 cells also make it a potential chemotherapeutic agent for breast cancer treatment.

Highlights

Nanotechnology is the one of up-to-date workspaces, which is aiming to develop materials with a range of 1–100 nm dimensions
Synthesized and characterization of synthesized iron oxide nanoparticles (IONPs) In recent years, plant extracts are more preferred in nanoparticle synthesis due to their inexpensive and environmentally friendly nature
To determine the effect of FeCl3 concentration on nanoparticle synthesis, concentration of Brassica oleracea var. capitata sub.var. rubra extract was fixed for reaction

Summary

Introduction

Nanotechnology is the one of up-to-date workspaces, which is aiming to develop materials with a range of 1–100 nm dimensions. Chemical, and green synthesis methods are used extensively for the production of metal oxide nanoparticles. Toxic chemicals such as sodium borohydride and hydrazine hydrate are used as reducing agents, which can cause severe harm to the nature and to the alive [11,12,13]. On account of these disadvantages of chemical and physical methods, green synthesis is proposed to be an appropriate alternative to these methods. These bioactive compounds can reduce positive charged metal ions and stabilize the nanoparticles to intended sizes and shapes [14,15]

Methods

Results

Conclusion