Abstract
Zinc Oxide nanoparticles (ZnO NPs) were synthesized through both chemical and green approaches using wheat grass (WG) extract. Characterization techniques included Dynamic Light Scattering (DLS), Scanning Electron Microscopy (SEM) with Energy-Dispersive X-ray Spectroscopy (EDX), Fourier-transform infrared Spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and Ultraviolet-visible (UV-Vis) spectroscopy. CS-ZnO NPs and WG-ZnO NPs exhibited characteristic surface plasmon resonance (SPR) peaks at 360 nm and 320 nm, respectively, with low polydispersity index (PDI) values indicating uniform nanoparticle dispersion. FTIR analysis revealed distinct functional groups on the ZnO NPs’ surface for stabilization. XPS confirmed atomic composition and oxidation state, while XRD confirmed their hexagonal wurtzite structure. SEM images revealed rod-shaped structures, supported by EDX analysis confirming ZnO NP formation through elemental content. The synthesized ZnO NPs demonstrated significant cytotoxicity against MDA-MB 231 breast cancer cells, causing changes in nuclear morphology, increased reactive oxygen species levels, and membrane damage. Wound healing assay highlighted their potential in inhibiting cancer metastasis. Molecular docking between WG compounds and breast cancer targets revealed that the enhanced efficacy of green-synthesized ZnO NPs could be attributed to covalent linkage of active molecules of WG onto the nanoparticle surface. Overall, ZnO NPs synthesized using the green method outperformed their chemically synthesized counterparts.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call