Abstract
High efficient enzyme immobilization on carbon based conductive supports could provide wide applications in energy and environmental science. Here, we synthesized a 3D flower-like structured self-assembly hybrid nanocomposite with copper phosphate, laccase, graphite oxide (GO) and carbon nanotubes (CNTs) via a facile one-pot strategy under mild conditions. The prepared nanocomposite exhibited very high enzyme loading and improved laccase activity. During the formation of the nanocomposite, the copper phosphate-laccase petals were interwined by CNTs, and GO nanosheets were further coated on the petal surface. Such a configuration ensured high enzyme loading between the GO sheets and good mass transfer efficiency between immobilized enzyme and substrate, which was confirmed by the kinetics test. We further deposited the immobilized enzyme onto electrodes and observed significantly improved direct electron transfer efficiency. Furthermore, higher dye removal efficiency was observed with the immobilized enzyme. The highly efficient enzyme immobilization strategy provides significant opportunity for its application in bioelectronics and wastewater treatment.
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