Abstract
In present study, a low-cost and steady electrochemical biosensor has been developed based on graphene oxide nanoparticles (GONPs), electrodeposited on the pencil lead electrode (PLE). The physical and morphological studies revealed the nano-scale range of GONPs that has an average grain size/layer thickness of 2.27 nm and agglomeration size of 90-120 nm. Urease enzyme was immobilized on PLE/GONPs electrode after surface treatment with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC)/N-hydroxysuccinimide (NHS) to impart stable peptide bond. Scanning electron microscopy (SEM) studies demonstrate the difference between unmodified and modified PLE electrodes and depict the spherical, circular and flaky-like morphology. The electrochemical analysis of urea considerably improved on the PLE/GONPs/Ur compared to the untailored PLE electrode. The reported urea biosensor operated in a linear dynamic range between 0.30-50 mM that attained a recognition limit of 0.06 mM and exhibited an advanced exposure sensitivity of 0.814 μA mM-1 cm-2.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
