Abstract
Glutathione is a natural antioxidant in human body and the depletion of its content commonly used as a marker for oxidative stress condition. In this study graphene modified screen printed carbon electrode (SPCE-G) was developed as working electrode for voltammetric detection of glutathione. Graphene was synthesized using microwave with nitronium ion, while deposition of graphene on to SPCE was performed by electrodeposition methods. Glutathione measurement was carried out by cyclic voltammetry using 1 mM NaBr solution in 0.2 M NaClO4 as electrolyte. The result indicated that graphene was successfully synthesized and deposited on to SPCE. The voltammetric measurement of glutathione based on oxidation of bromide in NaClO4 at SPCE-G provided a linear response with a detection limit (LOD) of 8.01 µM and %RSD of 2.95%. Despite further optimization is required to improve its analytical performances, graphene modified SPCE seems to be potential for voltammetric detection of glutathione.
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