Abstract
Robinia pseudoacacia L. is a tree widely dispersed in France that is characterized by good growth rates and important biomass production, which produces wood with very high natural durability used for outdoor fence posts, timber, and barrels to age vinegars and wines. Its mature heartwood presents high resistance against wood fungi decay and contains two main flavonoid extractives, dihydrorobinetin—the most abundant—and robinetin that present interesting biological activities. The aim of the present study was to optimize a procedure allowing an important recovery of purified dihydrorobinetin from R. pseudoacacia wood, representing an interesting sustainable, local, highly available, and, consequently, economical source of bioactive components. The extraction of dihydrorobinetin was first optimized by evaluating the influence of various extraction parameters such as temperature, extraction time, solvent nature, and wood/solvent mass ratio to obtain an efficient, safe, and low cost extraction. Then, dihydrorobinetin was purified over 95% using centrifugal partition chromatography (CPC). CPC purification was first developed on a small volume column with low amounts of injected extract, then scaled-up on a 200 mL column with higher sample loading capacity in order to purify more than 1.3 g of dihydrorobinetin in one run.
Highlights
Black locust (Robinia pseudoacacia L.) belongs to the Fabaceae family
Its rapid growth rate and the production of a large quantity of prolifically dispersed propagules allow black locust to successfully establish itself over a wide range of environmental conditions
We focused on dihydrorobinetin (DHRob) which is the main constituent of this resistant heartwood and that could be eventually valorized as a natural phytochemical protective for many applications in various activity sectors
Summary
Black locust (Robinia pseudoacacia L.) belongs to the Fabaceae family. Originating from southeasternNorth America this leguminous tree species was introduced in France in 1601 by Jean Robin and has since extensively spread within the temperate regions of North America, Europe, SouthernAfrica, and Asia. Black locust (Robinia pseudoacacia L.) belongs to the Fabaceae family. North America this leguminous tree species was introduced in France in 1601 by Jean Robin and has since extensively spread within the temperate regions of North America, Europe, Southern. With more than 3.2 million hectares, it is the third species used for wood production after poplars and eucalyptus [1]. Its rapid growth rate and the production of a large quantity of prolifically dispersed propagules allow black locust to successfully establish itself over a wide range of environmental conditions. R. pseudoacacia increases soil nitrogen stores [2]. It has been included on the European list of the most dangerous invasive species due to its significant regeneration abilities and its fast growth rate [3]
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