Abstract
Objectives: We hypothesized that the organisms and their organs or tissues could adapt themselves to the gradual changes of environment for surviving or reducing damage. This study explored whether gradual clamping (GC) could reduce myocardial ischemia-reperfusion (IR) injury in rat heart. Methods: Twelve rats were randomized to IR group and GC group, then the hearts were isolated and perfused with Langendorff apparatus. Before cardioplegia, the perfusion was stopped abruptly in IR group while slowly with 5-minute in GC group. The hearts were subjected to 30-minute ischemia and 60-minute reperfusion. The left ventricular develop pressure (LVDP) and systolic pressure (LVSP), the maximal rate of the increase and decrease of left ventricular pressure (+dp/dtmax, ﹣dp/dtmax) were measured by polygraph system at different time points. The recovery of the variables was expressed as the ratio of these values at individual time point after reperfusion to the baseline respectively. Results: The recovery of LVDP after reperfusion was better than that in IR group (P = 0.034). No significant difference in the recovery of LVSP, +dp/dtmax and ﹣dp/dtmax between groups was observed. Conclusions: Gradual clamping could improve the recovery of LVDP after IR, suggesting that gradual clamping could reduce myocardial IR injury.
Highlights
Cardiopulmonary bypass (CPB), considered as one of the most important clinical advances in medicine in theHow to cite this paper: Feng, H.B., Wang, H.L., Zhao, Y., Zheng, Z.N. and Jin, S.Q. (2016) Gradual Clamping Reduced Ischemia-Reperfusion Injury in an Isolated Rat Heart Model
According to the above hypothesis, we further hypothesized that gradual clamping, which means that the perfusion of the heart is blocked gradually at a constant speed till to cessation of the perfusion, but not ceased abruptly, could induce the myocardial gradual adaptation to ischemia, and so could reduce the myocardial ischemia-reperfusion injury
This study aimed to explore whether gradual clamping could reduce myocardial IR injury in isolated Langendorff-perfused rat heart
Summary
Cardiopulmonary bypass (CPB), considered as one of the most important clinical advances in medicine in theHow to cite this paper: Feng, H.B., Wang, H.L., Zhao, Y., Zheng, Z.N. and Jin, S.Q. (2016) Gradual Clamping Reduced Ischemia-Reperfusion Injury in an Isolated Rat Heart Model. (2016) Gradual Clamping Reduced Ischemia-Reperfusion Injury in an Isolated Rat Heart Model. CPB can cause myocardial ischemia-reperfusion (IR) injury which is closely associated with postoperative complications such as myocardial infarction and cardiac failure [2] [3]. Ischemic preconditioning (IPC) and remote ischemic preconditioning (RIPC) have the potential to attenuate IR injury [4] [5], their protective effects in clinic are still controversial and limited [6]-[8]. According to the above hypothesis, we further hypothesized that gradual clamping, which means that the perfusion of the heart is blocked gradually at a constant speed till to cessation of the perfusion, but not ceased abruptly, could induce the myocardial gradual adaptation to ischemia, and so could reduce the myocardial ischemia-reperfusion injury
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