Abstract
THE ISOLATION OF a protein believed to be pure metakentrin (ICSH) was described in 1940 (1). In a preceding report (2) the latest method of preparation was described in detail and the results of experiments indicating that hormone activity and the pure protein are inseparable were discussed. Immunological methods offer an additional means of demonstrating the specificity (uniqueness) and purity of the isolated protein. Application of these methods as here described demonstrated that hog metakentrin can readily be distinguished immunologically not only from sheep pituitary extract, sheep metakentrin (ICSH) or beef pituitary extract but also from extracts of hog tissues such as muscle, neural and intermediate lobes of the pituitary and even anterior pituitary extract, purified thylakentrin (FSH). Preparation of source material. The hormone protein used as antigen was isolated from hog pituitary glands, according to the procedure described in the preceding paper (a).
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