Gonadotrophin-induced follicle development in red deer hinds during the breeding and non-breeding seasons

Abstract

The effect of exogenous equine chorionic gonadotrophin (eCG) and endogenous (GnRH-treatment) gonadotrophins in promoting antral follicle development in red deer hinds was assessed during the breeding and non-breeding seasons. After progesterone pretreatment, hinds received no further treatment, a single injection of 300 iu equine chorionic gonadotrophin or infusion of GnRH (1.0 microg h(-1)) for up to 4 days. Ovaries were recovered (n = 5 per group) at the time of, or 36 h after, progesterone removal. All follicles > or = 2.0 mm in diameter were dissected out, their health status assessed and follicular fluid oestradiol content measured. Granulosa cells were counted and their capacity for oestradiol and cAMP production assessed in vitro. Oestrus, the preovulatory LH surge and ovulation rates were monitored in additional groups of hinds that had been treated identically (n = 5 per group). During the breeding season, all monitored animals ovulated, but five of 15 (one eCG, three GnRH, one progesterone alone) did not exhibit oestrus, and three (all eCG) had luteinized follicles. During seasonal anoestrus, four hinds (all eCG-treated) displayed oestrus, but only two ovulated. Two non-ovulating hinds (one eCG, one progesterone alone) had luteinized follicles. The total number of follicles, or of large (> or = 4 mm) follicles, did not differ significantly between seasons. There were proportionately more healthy follicles during seasonal anoestrus (P < 0.05). Treatment with GnRH, and to a lesser extent eCG, increased the number of oestrogenic follicles and their follicular fluid oestradiol content. In summary, exogenous and endogenous gonadotrophins affected antral follicle development similarly in both the breeding and non-breeding seasons, although the response was variable among animals and often associated with failure of oestrous expression, ovulation and with follicle luteinization.