Gold nanoparticle mediated delivery of fungal asparaginase against cancer cells

Abstract

The fungal asparaginase was immobilized on to gold nanoparticles for improved drug delivery in cancer treatment. FTIR and H NMR analysis of the synthesized nanobiocomposite showed that primary, secondary amines and allylic carbon were the main functional groups involved in the binding of asparaginase on to gold nanoparticle. The crystalline nature of the gold nanobiocomposite of asparaginase was confirmed by XRD pattern. The elemental analysis by EDS proved the presence of reduced metallic nanoparticles. The size of the nanobiocomposites ranged from 20 to 50 nm. The AFM displayed the smooth surface morphology of the nanoparticle. The specific activity of asparaginase was increased from 252.05 U/mg of crude asparaginase to 364 U/mg of gold nanobiocomposite of asparaginase. The protein concentration was increased from 0.018 mg/ml in crude asparaginase to 0.332 mg/ml in gold nanobiocomposite of asparaginase. The cytotoxicity effect of nanobiocomposite was found higher against lung cancer cell line A549 than ovarian cancer cell line A2780. The synthesized gold nanobiocomposite of asparaginase can be used as an effective anticancer drug with increased bioavailability against lung cancer cell line A549, providing toxicity of 84.51%.