Gold nanoclusters – 2D MoS2 nano couple: An immunosensor for the fluorescence turn-on detection of heart failure Biomarker Brain Natriuretic Peptide (BNP)

Abstract

In the context of elevated morbidity and mortality risk of heart failure (HF), the establishment of prompt and sensitive diagnosis of HF is vital. A nanostructured fluorescence immuno sensing probe was constructed out of noble metal nanoclusters / two-dimensional transition metal dichalcogenide nano couple to target brain natriuretic peptide (BNP) antigen, a potential multi-utility heart failure biomarker. The green fluorescence of BNP-specific monoclonal antibody-conjugated gold nanoclusters was pre-quenched by a MoS2 two-dimensional nanosheet, which is subsequently enhanced in the presence of BNP antigen via target-specific antigen–antibody interaction. The probe is free from interferences by other potentially interfering possible co-existing biomolecules and offers easy, low-cost linear detection of BNP in the concentration range 0 to 360.82 pg/mL.