Go green with plant organelle genome editing

Abstract

Programmable genome-editing technologies that enable efficient and precise genetic manipulation at targeted genomic regions, including zinc finger nucleases, transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat (CRISPR) systems, have become invaluable tools for plant biotechnology and precision breeding (Sedeek et al., 2019). Genome-editing systems have been utilized in a variety of plant species to characterize nuclear gene functions and improve agricultural traits, such as crop yields, nutritional values, and biotic and abiotic stress tolerance (Sedeek et al., 2019).