Glycyrrhizic Acid’s Effect on the Proliferation and Apoptosis of Thyroid Cancer Cell SW579 via LncRNA RP11-385J1.2-Targeted miR-370-3p

Abstract

This study assesses glycyrrhizic acid’s effect on thyroid cancer cell SW579. Thyroid cancer cell lines were selected to detect RP11-385J1.2 and miR-370-3p level by QRT-PCR. Cells were treated with glycyrrhizic acid followed by measuring cell apoptosis by flow cytometry and cell proliferation by MTT assay and protein expression by western blot. Under glycyrrhizic acid (40 μM) treatment, the proliferation of SW579 cells was weakened and apoptosis increased significantly (P < 0.05) along with reduced RP11-385J1.2 and increased miR-370-3p expression compared to untreated (P < 0.05). miR-370-3p has a targeting relationship with RP11-385J1.2. RP11-385J1.2 overexpression significantly reduced miR-370-3p, which can reverse glycyrrhizic acid’s effect on inhibiting cell proliferation and promoting apoptosis. RP11-385J1.2 overexpression reversed the effect of glycyrrhizin on cell proliferation and apoptosis, and inhibition of miR-370-3p reversed si-RP11-385J1.2’s effect on inhibiting cell proliferation and promoting apoptosis (P <0.05). In conclusion, glycyrrhizic acid targets miR-370-3p through lncRNA RP11-385J1.2 to inhibit thyroid cancer cell proliferation. It participates in the development of tumor cells, and lncRNA RP11-385J1.2 and miR-370-3p is negatively correlated, providing new experimental data and evidence for treating thyroid cancer.