Abstract
The sequential glycosylation of a soybean isoflavone, daidzein, with cultured suspension cells of Eucalyptus perriniana and cyclodextrin glucanotransferase was studied. Daidzein was converted into two glycosylation products, daidzein 7- O-β- d-glucopyranoside (39%) and daidzein 7- O-[6- O-(β- d-glucopyranosyl)]-β- d-glucopyranoside (β-gentiobioside, 6%), by cultured E. perriniana cells. Further glycosylation of daidzein 7- O-β-glucoside with cyclodextrin glucanotransferase gave daidzein 7- O-[4- O-(α- d-glucopyranosyl)]-β- d-glucopyranoside (β-maltoside, 26%), daidzein 7- O-β-maltotrioside (15%), and daidzein 7- O-β-maltotetraoside (7%).
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